Table 1. Primary Antibodies.
| Antiserum | Immunogen | Source(cat. no.) | Working dilution | Specificity |
| Rabbit polyclonalanti-internexin,alpha | Full-length recombinantrat alpha-internexinfused to E. Coli Trp E. | Chemicon International,Temecula, CA (AB5354) | 1∶250 (I)1∶1000 (W) | Alpha internexin(66 KDa) |
| Rabbit polyclonalanti-laminin | Protein purified fromthe basement membraneof Englebreth Holm-swarm (EHS) sarcoma (mouse) | Abcam (ab11575) | 1∶800 (I) | Laminin is composed of one A chain (400 KDa), one B1 chain (215 KDa), |
| Anti-myosineHeavy chainType 1 | MyHC (Human)partially purified, pyrophosphate extracted (22 year quadriceps) | Developmental StudiesHybridoma Bank(A4.840) | ud (I) | Adult myosin (200 KDa) |
| Anti-myosineHeavy chainType 2A | Purified myosinorigin from bovinesubcutaneous muscle | Developmental StudiesHybridoma Bank(SC-71) | ud (I) | Immunoblotting (2A myosin heavy chain) |
| Anti-myosineHeavy chainType 2B | Purified myosinbovine skeletal muscle(fetal, 3 months) | Developmental StudiesHybridoma Bank(BF-F3) | ud (I) | Recognized 2B myosinheavy chain |
| Anti-myosineHeavy chainType 2X | Myosin co-absorbedwith an adjuvant peptide to gold particles rabbit retractor bulbi muscle | Developmental StudiesHybridoma Bank(6H1) | ud (I) | Immunoblotting (2Xmyosin heavy chain) from rat and rabbit limb muscles (200 KDa) |
| Mousemonoclonal anti-neuron-specific nuclear protein (NeuN) | Purified cell nuclei frommouse brain | Chemicon International,Temecula, CA(MAB377B) | 1∶100 (I)1∶200 (W) | It recognizes 2-3 bandsin the 46–48 KDa range(manufacturer's technical information). |
| Mousemonoclonal anti-neurofilament RT-97 specific for phosphorylated NF-H | Wistar ratneurofilaments | Developmental StudiesHybridoma Bank (RT-97) | 1∶3 (I) | It recognizes a band at 200 KDa corresponding to NF-H protein (manufacturer's technical information). |
| MouseMonoclonal anti-synaptic vesicle 2 (SV2) | Synaptic vesicleProtein 2 | Developmental StudiesHybridoma Bank (SV2) | 1∶100 (I) | It recognizes a band at∼95 KDa (manufacturer's technical information) |
| MouseMonoclonal anti-dystrophin (C-terminus, clone Dy8/6C5) | Synthetic polypeptide re-presenting the last 17 amino acids at the car-Boxy terminus of the human dystrophin molecule (SSRGRNTPGKPMREDTM) | Vector Laboratories(VP-D505) | 1∶20 (I)1∶10–1∶25 (W) | Skeletal muscle, singleStrong band at ∼400 KDa (manuf0acturer's technical information). |
| Mousemonoclonal anti-pan-axonal neurofilaments | Pan-axonalneurofilament | Covance(SMI-312R) | 1∶1000 (I)1∶1000 (w) | SMI-312 is a specific marker for axons in tissue sections and cultures. |
(I), Immunohistochemistry, (W), Western blot, ud (undiluted).