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. 2011 Jun 15;6(6):e21136. doi: 10.1371/journal.pone.0021136

Figure 4. Morphological and immunohistochemical characteristics of hESC derived FRT epithelial structures in grafted GF treated recombinants.

Figure 4

(A) H&E showing pseudostratification in the hESC derived FRT epithelium surrounded by co-transplanted mouse stroma in a 4 week graft. (B) no PAX2 or HOXA10 (C) expression in week 2 hESC derived FRT epithelium (B, C) were serial sections representative of two week 2 graft) (arrows point to HOXA10+ mouse stromal cells). (D–I) Representative sections from two different 4 week grafts, (E–J) serial sections of a single gland (sectioned at 3 µm), (D, E) Nuclear expression of HOXA10 and PAX2 in hESC derived FRT epithelium at 4 weeks. (F) representative serial sections of the same gland depicted from (E–J) showing GFP+ hESC derived epithelium surrounded by GFP− mouse uterine stromal cells. The gland co-localised cytoplasmic expression of (G) CK18 and (H) Vimentin in hESC derived FRT epithelium. (I) CA125 expressed on hESC derived FRT epithelium surface. (J) ERα expressed in transplanted mouse stromal cells but absent from hESC derived FRT epithelium. Insets in (B) is a positive control of PAX2 expression in adult human endometrial epithelium, inset in (C) concentration matched goat IgG negative control, inset in (E) concentration matched rabbit IgG negative control, inset in (I) mouse IgG1 negative control for (G–I). (K.1–2) 3D reconstruction of hESC derived FRT epithelium from serial sections depicted in (E–J) (arrow) shown on the Y-axis, (K.2) shown on the Z-axis view as a duct. Abbreviations: E, epithelium; hFRT, hESC derived FRT; HOXA10, Homeobox A10; PAX2, Pair box gene 2; S, stroma.