Table 1. Primers used for mRNA quantification by real-time RT-PCR.
| Gene | Forward primer 5′-3′ | Reverse primer 5′- 3′ |
| mLDL-R | TGACTCAGACGAACAAGGCTG | ATCTAGGCAATCTCGGTCTCC |
| mHMG-CS | AACTGGTGCAGAAATCTCTAGC | GGTTGAATAGCTCAGAACTAGCC |
| mHMG-CR | CTTGTGGAATGCCTTGTGATTG | AGCCGAAGCAGCACATGAT |
| mFAS | GCTGCGGAAACTTCAGGAAAT | AGAGACGTGTCACTCCTGGACTT |
| mACS | GCTGCCGACGGGATCAG | TCCAGACACATTGAGCATGTCAT |
| mSCD-1 | CCGGAGACCCCTTAGATCGA | TAGCCTGTAAAAGATTTCTGCAAACC |
| mβ-actin | GGCTGTATTCCCCTCCATCG | CCAGTTGGTAACAATGCCATGT |
| hLDL-R | AGCTGCCTCACAGAGGCTGA | CCAGAGCTTGGTGAGACATTG |
| hHMG-CS | TGTTTCAGATTGCAAGGTAATATGT | CCTGGCACCCAAAAGTTAAA |
| hHMG-CR | TACCATGTCAGGGGTACGTC | CAAGCCTAGAGACATAATCAT |
| hFAS | AACTCCAAGGACACAGTCACCAT | CAGCTGCTCCACGAACTCAA |
| hACS | AACCACGAACGCTTTGAGA | TCCAGATACATTGAGCATGTCAT |
| hSCD-1 | TGTGTCCCAGATGCTGTCAT | CCCACCCAATCACAGAAAAG |
| hβ-actin | GGCATCCTCACCCTGAAGTA | AGGTGTGGTGCCAGATTTTC |
The abbreviations are: LDL-R, low-density lipoprotein receptor; HMG-CR, 3-hydroxy-3-methylglutaryl coenzyme A reductase; HMG-CS, 3-hydroxy-3-methylglutaryl coenzyme A synthetase; FAS, fatty acid synthetase; ACS, acetyl-CoA synthetase; SCD-1, stearoyl-CoA desaturase 1. The prefix m indicates mouse and h human sequence.