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. 2011 Jun 15;6(6):e21229. doi: 10.1371/journal.pone.0021229

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(A) Wild type and Car null mice were given DAS (80 mg/100 g body weight) by gastric gavage. B. Following 6, 24, 48, 72, and 96 hrs after chemical administration, the expression of Sult1e1, Cyp2b10, Cyp3a11, and Gadd45b genes was analyzed by quantitative PCR as described in the methods section. The relative mRNA amount of each gene was calculated so that wild type vehicle treated samples have one unit of expression. (B) Liver nuclear extracts from DAS treated mice at indicated time after the chemical administration were prepared and CAR protein content was analyzed by western blotting. Anti TATA binding protein (TBP) western blotting is shown as a loading control.