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. 2011 Jun 15;6(6):e21215. doi: 10.1371/journal.pone.0021215

Figure 2. AmiRs inhibit CVB3 induced cell death in AmiR-expressing cell lines.

Figure 2

A) Establishment of AmiR-expressing cell lines. Four stable cell lines over-expressing an individual AmiR were established by transfection of HeLa cells with the AmiR plasmids. cGFP tag expression was used as a selection marker. B) Western Blotting analysis for cGFP expression of the established AmiR cell lines. The cell lysates of these cell lines were collected and applied to Western blotting detection of cGFP expression. The non-transfected HeLa cell lysate served as a negative control. C) Cell morphology changes after CVB3 infection. Cell lines expressing different AmiRs were infected with CVB3 (Kandolf). Cell morphology was observed under a phase-contrast microscope. Dying cells appeared rounding and detachment. D) MTS assay of CVB3 infected AmiR cells. Cell lines expressing different AmiRs were infected with CVB3 as described above. Cell viability was measured by MTS assay. The survival values of the samples were converted to the ratio of the sham-infected HeLa cells, which were defined as 100%.