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. 2011 Jun 15;6(6):e21215. doi: 10.1371/journal.pone.0021215

Figure 9. Anti-CVB3 (CG) effect of pRNA-AmiR chimerics in HL-1 cells.

Figure 9

HL-1 cells were transfected with different pRNA-AmiRs or mock-transfected with Oligofectamine™ only for 48 h and then infected with CVB3 (CG, 10 MOI 20 h) or sham-infected with PBS. Cell morphologies were analyzed by phase-contrast microscopy. Dying cells appeared rounding and detachment (A). Cell viability was measured by MTS assay (B), the viral VP-1 protein was detected by Western blot analysis (C) and viral particle formation was measured by plaque assay (D).