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. Author manuscript; available in PMC: 2012 Mar 1.
Published in final edited form as: Anal Biochem. 2010 Nov 10;410(1):133–140. doi: 10.1016/j.ab.2010.11.004

Fig. 1.

Fig. 1

Schematic of the fluorescent HCS assay. The CoA produced in the HCS reaction is quantified through its reaction with the sulfhydryl-sensitive fluorophore MMBC (also known as ThioGlo 1). The reaction yields an MMBC-CoA adduct that fluoresces strongly at 470 nm when excited at 380 nm.