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. Author manuscript; available in PMC: 2012 Mar 1.
Published in final edited form as: Anal Biochem. 2010 Nov 10;410(1):133–140. doi: 10.1016/j.ab.2010.11.004

Fig. 4.

Fig. 4

Control assays for the metal-chelation counter screen. (A) SpHCS activity in the presence of increasing concentration of MnCl2. The fraction activity was calculated relative to the activity of the enzyme in the absence of MnCl2. The averages of three measurements with an error of one SD are illustrated. (B) Fractional activity of SpHCS incubated with 0 or 10 μM MnCl2 in the presence of increasing concentrations of EDTA. Data represent the average of triplicate measurements normalized to SpHCS activity in the absence of MnCl2 and EDTA with error bars indicating one SD.