Table 7.
Numbers and types of structural aberrations detected by PAINT/DAPI analysis in mouse zygotes after exposure of male mice to ET
| Dose, mg/kg | Days post ET | DAPI
analysis
|
PAINT analysis*
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Total cells | Dicentrics | Fragments
|
RFL† | Other | Cell eq‡ | Translocations
|
dic(AB) | Acentric
fragments
|
|||||
| Acentric | Centric | t(Ab) | t(Ba) | ace(ab) | ace(b) | ||||||||
| 0 | 318 | 0 | 2 | 0 | 0 | 0 | 114 | 0 | 0 | 0 | 0 | 0 | |
| 80 | 6.5 | 180 | 0 | 1 | 0 | 0 | 1 | 63 | 0 | 0 | 0 | 0 | 0 |
| 80 | 24.5 | 254 | 9 | 33 | 8 | 12 | 3 | 94 | 4 | 1 | 1 | 5 | 9 |
| 80 | 34.5 | 146 | 3 | 11 | 0 | 0 | 1 | 54 | 0 | 1 | 3 | 0 | 0 |
| 80 | 41.5 | 152 | 1 | 2 | 1 | 0 | 1 | 57 | 0 | 1 | 0 | 0 | 1 |
A detailed description of these aberrations can be found in Marchetti et al. (31).
Robertsonian fusion-like chromosomes.
Cell-equivalent (number of cells analyzed/percent of exchanges that can be detected with our probe combination).