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. Author manuscript; available in PMC: 2012 Jan 1.
Published in final edited form as: J Invest Dermatol. 2011 Mar 31;131(7):1579–1583. doi: 10.1038/jid.2011.65

Figure 2. Activated BRAF promotes melanoma cell growth by MMP-1.

Figure 2

(a) Relative MMP-1 mRNA levels in HPMs and melanoma cells expressing wildtype (WM852), or mutant BRAF (WM793). (b) Relative levels of secreted MMP-1 in conditioned media obtained from HPMs expressing GFP or BRAFV600E at 72 hours following lentiviral infection. (c) Relative MMP-1 collagenase activity in conditioned media obtained from HPMs expressing GFP or BRAFV600E at 72 hours following lentiviral infection. (d) qRT-PCR analysis of MMP-1 expression following gene silencing by siRNA in melanomas possessing either wildtype (WM852) or mutant BRAFV600E (WM793). (e) Relative MMP-1 concentration in cell culture media following MMP-1 gene silencing in melanomas possessing wildtype (WM852) and BRAFV600E (WM793) cells. (f). 3H-thymidine cell proliferation assay of melanomas possessing wildtype (WM852) and BRAFV600E (WM793) following MMP-1 gene silencing. (g) Relative expression of activated AREG in conditioned media from HPMs expressing GFP or BRAFV600E. (h) Relative expression of activated AREG in melanomas expressing wildtype (WM852), or mutant BRAF (WM793) following MMP-1 gene silencing. Columns, mean of three individual experiments done in triplicate; bars, SD. *, P < 0.05, **, P < 0.01, ***, P < 0.001, compared with GFP control in the Figure 2b, c, g, and compared with siRNA control (Scramble) in the Figure 2d, e, f, h.