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. Author manuscript; available in PMC: 2012 Jun 15.
Published in final edited form as: Melanoma Res. 2011 Feb;21(1):35–43. doi: 10.1097/CMR.0b013e3283426805

Figure 3. Murine Anti-GRP78 Autoantibodies.

Figure 3

Panel A. Endpoint titers of mouse sera taken before tumor implantation. Samples were diluted and then assayed on plates coated with rGRP78. Endpoints were defined as the highest dilution yielding absorbance threefold higher than background. Panel B. ELISA data from adjuvant-only (N=9) and GRP78-immunized (N=10) mice. EIA/RIA plates were coated with CNVSDKSC peptide conjugated to KLH. Sera were diluted 1:100 in 1%BSA and incubated overnight at 4°C. Data are presented as absorbance units at 450nm and P value here is derived from the nonparametric Mann-Whitney test.