Figure 1.
(a) Mechanistic proposal for the formation of the thiamin thiazole in B. subtilis. The thiazole synthase (ThiG) reaction product 14 is coupled to HMP-PP 17 to form thiamin phosphate 18. Thz-P 15 and cThz-P 16 are possible intermediates in this coupling reaction. (b) Reactions showing conversion of ThiS by ThiF and NifS into ThiS-COSH 7, glycine oxidase ThiO converting glycine 19 to glycine imine 12 and the oxidation of thiamin phosphate 18 to thiochrome 20.