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. 2011 Mar 10;29(5):847–857. doi: 10.1002/stem.627

Figure 5.

Figure 5

Verification of miRNA-mRNA interactions via luciferase assay. Cotransfection of pMIR-Luc-FZD5 and 100 nM miR-29a decreased 41% of the luciferase activity in comparison with the blank control. Cotransfection of pMIR-Luc-TPM and miR-29a decreased the luciferase activity by 33% and pMIR-Luc-CD133 and miR-142-3p by 30%. For each cotransfection, 300 ng vector and 50 nM (miR-142-3p) or 100 nM (miR-29a) oligonucleotide were used. Relative luciferase activity represents firefly luciferase activity normalized versus renilla activity. Data are representative of at least five independent experiments. miR-Neg, oligonucleotide designed to serve as negative control.