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. 2011 Apr 6;41(6):1652–1662. doi: 10.1002/eji.201041120

Figure 6.

Figure 6

Tr1-mediated cytotoxicity requires CD18 adhesion and activation via CD2 and CD226. (A) Tr1-cell lines were co-cultured with freshly isolated allogeneic CD14+ and CD1c+ cells at 10:1 (E:T) ratio in the presence of anti-CD18 mAb or IgG1 isotype control, and degranulation in the presence of GZB was measured by co-expression of CD107a and GZB in CD4+ T cells. One donor representative of three donors tested in two independent experiments is shown. Numbers represent percentage of CD107a+GZB+ cells. (B) Tr1- and Th0-cell lines were co-cultured with p815 cells pre-incubated with anti-CD2 mAb at 10:1 (E:T) ratio, and degranulation was determined by co-expression of CD107a and GZB in CD4+ T cells. One donor representative of three donors performed in two independent experiments is shown. Numbers represent percentage of CD107a+GZB+ cells. (C) Tr1- and Th0-cell lines were co-cultured with p815 cells pre-incubated with anti-CD226 mAb at 10:1 (E:T) ratio, and degranulation was determined by co-expression of CD107a and GZB in CD4+ T cells. One donor out of five donors performed in two independent experiments is shown. Numbers represent percentage of CD107a+GZB+ cells.