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. 2011 Apr 28;12(5):2783–2796. doi: 10.3390/ijms12052783

Table 1.

Treatment of lymphocytes and sperm with 5 oestrogenic compounds, H2O2 and the negative control with and without the presence of FPG (1:3000) and Endo III (1:3000). The table expresses the mean of the values obtained from 7 independent experiments (n = 7 different donors) for lymphocytes and 5 independent experiments (n = 5 different donors) for sperm. For lymphocytes the average age was 27.57 ± 2.41. For sperm the average age was 30.8 ± 2.35. The data were evaluated for normality with the Shapiro-Wilks test. The data were normally distributed. ANOVA with Dunnet’s post-hoc was applied. For statistical interpretation of data, treatments with FPG or EndoIII were compared to the corresponding control.

Compound Treatment Lymphocytes Sperm
120 min treatment 120 min followed by 24 h repair (Olive tail moment) 120 min (Olive tail moment)
Control Buffer 3.65 ± 0.28 1.89 ± 0.32 2.94 ± 0.24
FPG 6.29 ± 0.85 (**) 2.34 ± 0.72 (n.s.) 3.96 ± 0.34 (**)
EndoIII 6.36 ± 0.55 (**) 2.08 ± 1.65 (n.s.) 4.12 ± 0.36 (**)
H2O2 50 μM 17.13 ± 1.22 5.44 ± 1.36 4.98 ± 0.40
FPG 16.72 ± 1.21 (n.s.) 6.51 ± 1.71 (n.s.) 4.91 ± 0.52 (n.s.)
EndoIII 18.45 ± 1.16 (n.s.) 5.94 ± 1.80 (n.s.) 5.03 ± 0.47 (n.s.)
17 β-oestradiol 100 μM 8.01 ± 1.72 2.51 ± 0.70 5.52 ± 0.67
FPG 8.89 ± 1.43 (n.s.) 2.97 ± 0.73 (n.s.) 6.24 ± 1.07 (n.s.)
EndoIII 9.04 ±1.57 (n.s.) 3.01 ±0.65 (n.s.) 3.65 ± 0.50 (**)
Progesterone 100 μM 7.81 ± 1.34 2.76 ± 0.64 6.35 ± 0.57
FPG 7.82 ± 1.23 (n.s.) 2.89 ± 0.63 (n.s.) 5.44 ± 0.65 (n.s.)
EndoIII 7.04 ±1.09 (n.s.) 2.94 ±0.53 (n.s.) 5.82 ± 0.92 (n.s.)
DES 100 μM 22.62 ± 3.73 3.03 ± 0.86 6.55 ± 0.81
FPG 24.90 ± 4.22 (n.s.) 4.80 ± 1.30 (n.s.) 5.48 ± 0.56 (n.s.)
EndoIII 24.01 ± 3.24 (n.s.) 3.28 ± 1.56 (n.s.) 4.92 ± 0.75 (n.s.)
T3 46.9 μM 6.63 ± 1.03 2.02 ± 0.29 4.11 ± 1.12
FPG 6.99 ± 1.31 (n.s.) 2.53 ± 0.33 (n.s.) 4.12 ± 0.43 (n.s.)
EndoIII 6.09 ± 0.94 (n.s.) 2.28 ± 0.26 (n.s.) 4.45 ± 0.89 (n.s.)
Noradrenaline 100 μM 13.08 ± 2.91 2.72 ± 0.75 3.39 ± 0.70
FPG 13.04 ± 1.30 (n.s.) 3.93 ± 1.23 (n.s.) 3.25 ± 0.77 (n.s.)
EndoIII 13.50 ± 1.14 (n.s.) 2.78 ± 0.57 (n.s.) 2.79 ± 0.34 (n.s.)

The levels of significance are included: n.s. not significant;

*

p ≤ 0.05;

**

p ≤ 0.01 and

***

p ≤ 0.001. H2O2: hydrogen peroxide; DES: diethylstilboestrol; T3: triiodo-L-thyronine.