Table 3.
Status of NtN1-ELP | Total protein (mg) | Total activity (U) | Recovery rate (%) | Specific activity (U mg−1) |
---|---|---|---|---|
Raw extract | 3521.9 | 736.5 | 100 | 0.21 |
Purified NtN1-ELP by mITC | 20.3 | 681.1 | 92.5 | 33.6 |
Purified NtN1-ELP by cITC | 31.2 | 248.5 | 33.7 | 7.96 |
The NtN1-ELP activity was determined by fluorescence assay before and after purification by mITC or cITC. Data were calculated for 1 kg of leaf material.