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. 2001 Mar 20;98(7):3964–3969. doi: 10.1073/pnas.061507398

Figure 2.

Figure 2

Blocking of T cell recognition of NY-ESO-1 epitopes by an anti-DP Ab. 1088EBVB cells pulsed with 20 μg/ml ESO p161–180 peptide were used as target cells in the presence of different blocking antibodies. Specificity of Abs used was as follows: anti-MHC class I (HLA A, B, C) Ab (W6/32), anti-MHC class II (HLA DP, DQ, DR) Ab (IVA12), anti-HLA DP Ab (B7/21), anti-HLA DR Ab (L243), and anti-HLA DQ Abs [mixture of Genox 3.53 (anti-DQw1) and IVD12 (anti-DQw3)]. All Abs were used at a final concentration of 20 μg/ml each. CK3H6 T cells specifically recognized gp100 p209–218 peptide in the context of HLA A2 and was used as the specificity control for anti-MHC class I Ab. 1088EBVB (A2+) pulsed with gp100 p209–218 peptide was used as targets. A CD4+ T cell line (T3–80) recognized 1362mel in an HLA-DR1-restricted fashion and was used as the specificity control for anti-MHC class II and anti-DR Abs.