Yorkie is required for actin dynamics induced overgrowth and upregulation of Hippo target genes. (A–C) Adult flies of the indicated genotypes. (D) Quantification of the number of enclosed adult flies of the genotypes in (B, C) relative to (A). (E) Confocal image of a third instar wing imaginal disc stained for GFP (green, grey in E′), Yki (red, grey in E′′), and Dapi (blue, grey in E′′′) of hh-Gal4, UAS-GFP, UAS-cpaRNAi. (F, I) Third instar wing imaginal discs stained for GFP (green) and ex-lacZ (red, grey in F′–I′) of (F) hh-Gal4, UAS-GFP, UAS-ykiRNAi, (G) hh-Gal4, UAS-GFP, UAS-ykiRNAi, UAS-cpaRNAi, (H) dpp-Gal4, UAS-GFP, UAS-ykiRNAi, and (I) dpp-Gal4, UAS-GFP, UAS-ykiRNAi, UAS-diaCA.