Effect of CP-96,345 and/or anti-SP antibody on SP-enhanced HIV
replication. HIV Bal strain was used to infect MDM in the presence or
absence of SP (10−6 M), CP-96,345 (10−6 M),
or anti-SP antibody (1:1,000 dilution) as indicated. CP, CP-96,345. HIV
only, untreated and HIV Bal strain-infected MDM was used as a control.
HIV RT activity (A) in the culture supernatants was
determined 12 days after infection, and HIV gag mRNA
copy numbers (B) were quantified by using real-time
RT-PCR 12 days after infection (B). The data shown are
presented as the mean ± SD of triplicate cultures and are
representative of three experiments (*, P <
0.05; **, P < 0.01; treatment vs.
HIV only).