Effect of CP-96,345 on CCR5 mRNA expression in MDM. −, PCR negative
control, which represents lack of PCR products when RT was omitted from
the RT reaction by using RNA extracted from untreated MDM. MDM only,
untreated MDM was used as a control. MDM were incubated with CP-96,345
(10−8 to 10−6 M) as indicated for 3 h,
and CCR5 mRNA was then amplified by using RT-PCR. M, 100-bp DNA ladder
coelectrophoresed as markers. β-actin was used to monitor the amount
and integrity of RNA in each sample.