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. 2011 Jun 8;108(24):9957–9962. doi: 10.1073/pnas.1103132108

Fig. 5.

Fig. 5.

aPKCζ and Par6 are not polarized in HSC and aPKCs do not regulate HSC polarization. (A) Immunofluorescence pictures showing aPKCζ (red) along with tubulin (blue) localization in LT-HSCs. (Ai, Aii) aPKCζ is detected diffusely in the cell cytoplasm without any specific asymmetric distribution in most of LT-HSCs. (Aiii, Aiv) Most of LT-HSCs display a highly polarized distribution of the microtubule network. (Scale bar, 5 μm.) (B) Immunofluorescence pictures showing Par6 (green) along with tubulin (blue) localization in LT-HSCs. (Bi, Bii) Par6 is distributed throughout the cell body and beneath the cell membrane. Par6 localization is not polarized in most of LT-HSCs. (Biii, Biv) LT-HSCs display a highly polarized distribution of the microtubule network. (Scale bar, 5 μm.) (C) Bar diagram showing quantification of immunofluorescence analysis. Fifty to 100 LT-HSCs were singularly analyzed in each experiment (n = 4). Error bars represent SEM of the number of tubulin (79.7%), aPKCζ (21.53%), and Par6 (41.24%) polarized cells scored per sample. *P < 0.05. (D) Immunofluorescence pictures showing tubulin (blue) localization in LT-HSCs isolated from wild type or aPKCζ- and aPKCλ-deficient mice. Most of LT-HSCs display a highly polarized distribution of the microtubule network (n = 3–4 mice per group) (Scale bar, 5 μm.) (E) Bar diagram showing quantification of immunofluorescence analysis (n = 30–50 LT-HSCs from individual mouse). Error bars represent SD.