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. 2011 May 31;108(24):9951–9956. doi: 10.1073/pnas.1016912108

Fig. 1.

Fig. 1.

c-Met is expressed and functional in GBM-derived neurospheres. (A) Immunoblots showing activated (phosphorylated) c-Met in multiple human GBM-derived neurospheres. (B) Neurosphere cells were cultured overnight without EGF/FGF and then ± (with or without) HGF for 15 min. HGF induces c-Met, AKT, MAPK, and Stat3 activation (phosphorylation). (C) Neurospheres were treated ± HGF for 3 h. Immunofluorescent micrographs of neurosphere cytospins show HGF-induced translocation of Stat3 (green) to DAPI-stained nuclei (blue). (D) Mayo39 neurosphere cells grown in neurosphere medium containing EGF/FGF were treated ± the c-Met inhibitor SU11274 for 1 h. Immunoblots show that SU11274 inhibits basal c-Met, AKT, MAPK, and Stat3 activation (phosphorylation).