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In vitro polymerization of the C-terminal ChpH peptide (GNTISVIGLLNPAFGN). (A) ThT fluorescence of the peptide (0.5 mg/mL) over 120 h (24 h intervals). (B) CD analysis of the peptide (diluted from 0.5 to 0.25 mg/mL) from 0 to 120 h. (C–D) Negatively stained electron micrographs of the peptide (0.5 mg/mL) incubated at room temperature for (C) 72 h or (D) 96 h. (Scale bar, 250 nm).
