Table 1.
Assessment of nanoparticle formation by CXCR4 TM2 derivatives
| Peptide | Structure | Particle hydrodynamic radii, nm* (population weight fraction) | Percentage polydispersity† | Apparent number of peptide molecules per particle |
| X4-2-1 | LLFVITLPFWAVDAVANWYFGNDD-OH | 6.0 (87.3%) 22.4 (12.1%) | 6.7 16.1 | 80 1746 |
| X4-2-2 | LLFVITLPFWAVDAVANWYFGNKK-NH2 | 66.5 (100%) | 68.7 | 20235 |
| X4-2-3 | LLFVITLPFWAVDAVANWYFGN-OH | 92.2 (100%) | 19.5 | 49369 |
| X4-2-5 | LLFVITLPFWAVDAVANWYFGN-(CH2CH2O)27-NH2 | 6.8 (99.9%) | 26.2 | 80 |
| X4-2-4 | LLFVITLPFWAVDAVANWYFGNDD-(CH2CH2O)11-NH2 | 5.5 (100%) | 11.8 | 42 |
| X4-2-6 | LLFVITLPFWAVDAVANWYFGNDD-(CH2CH2O)27-NH2 | 5.5 (99.9%) | 8.5 | 45 |
| X4-2-11 | LLFVITLPFWAVDAVANWYFGNDD-(CH2CH2O)27-CO-NH-(CH2CH2O)11-NH2 | 5.6 (99.9%) | 16.5 | 41 |
| X4-2-15 | LLFVITLPFWAVDAVANWYFGNDDD-(CH2CH2O)27-NH2 | 5.3 (99.9%) | 12.5 | 40 |
| X4-2-9 | FVITLPFWAVDAVANWYFGNDD-(CH2CH2O)27-NH2 | 11.1 (97.4%) 62.8 (2.5%) | 16.9 19.5 | 302 21817 |
| X4-2-10 | TLPFWAVDAVANWYFGNDD-(CH2CH2O)27-NH2 | 1.6 (100%) | 15.5 | 3 |
| X4-2-8 | AAVANWYFGNDD-(CH2CH2O)27-NH2 | N/A | N/A |
*The particle hydrodynamic radii were determined from DLS. The data presented is for 0.4 mg/mL solutions of peptides in PBS containing 1.25% (v/v) DMSO. Dynamics 6.7.7.9 software (Wyatt Technologies) was used to calculate molecular weight from the size data. The calculation was based on a globular protein model. Comparison of the calculated apparent molecular weight with the weight of the monomer provided the apparent number of peptide molecules per particle.
†Percentage polydispersity reflects the homogeneity of the nanoparticles. It is defined as a standard deviation of the size divided by the mean radius multiplied by 100%.