Fig. 5.

Tumstatin and/or TSP1 deficiency affect the phenotype and the tumor spectrum of p53-deficienct mice. (A) Survival curves of p53^−/− (n = 14), p53^−/−/tumstatin^−/− (n = 11), and p53^−/−/tumstatin^−/−/TSP1^−/− (n = 7) mice. Deficiency of tumstatin and TSP1 significantly decreased the lifespan of p53-deficient mice. (B) Lymphomas from p53^−/−, p53^−/−/tumstatin^−/−, and p53^−/−/tumstatin^−/−/TSP1^−/− were examined for vessel number by CD31 immunofluorescence. (C) Vessels from the lymphomas were also analyzed for colocalization of CD31 with β3 integrin. Expression of the thrombospondin receptors CD36 (D; 200× magnification) and CD47 (E; 400× magnification) were also observed in lymphomas of p53^−/− mice. Results are shown as mean ± SEM; *P < 0.05, **P < 0.01. (Scale bar, 20 μM.) See also Fig. S3 and Table S1.