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. 2011 May 31;108(24):E211–E217. doi: 10.1073/pnas.1102637108

Fig. 5.

Fig. 5.

COPI coatomer protein function is required for induction of late-phase protease expression in blood-fed mosquitoes. (A) Western blot analysis of AaSPVI and AaSPVII serine protease protein expression 24 h PBM in midgut epithelial cells of blood-fed mosquitoes that had been injected with 400 ng indicated dsRNA species 4 d earlier. Protein loading was monitored using an anti-GAPDH antibody. (B) qRT-PCR analysis of AaSPVI and AaSPVII transcript levels 24 h PBM in midgut epithelial cells of blood-fed mosquitoes that had been injected with the indicated dsRNA species (400 ng) 4 d earlier. Data show protease transcript levels as the mean value ± SEM in 10 individual mosquitoes using ribosomal protein S7 transcript levels as the internal control. Student t test analysis showed that AaSPVI and AaSPVII transcript levels in α-, β-, β′-, γ-, δ-, and aνδ ζCOPI dsRNA-injected mosquitoes were significantly lower than in Fluc and εCOPI dsRNA-injected mosquitoes (P < 0.001).