Table 1.

Half-lives of uncoating and fusion in the CsA washout assay

Virus	Uncoating (min)	Uncoating + NVP (min)	Fusion (min)	Normalized uncoating (min)
HIV-GFP	39.12 (4.14)	117.87 (8.21)	15.81 (3.02)	23.31
HIV-GFPNevR	38.94 (4.02)	36.25 (3.13)	ND	ND
R7X4-GFP	73.5 (4.72)	142.57 (1.99)	48.33 (0.19)	25.17

Time after infection at which 50% of cells were GFP positive in the normalized CsA washout assay was calculated from multiple independent experiments and averaged for HIV-GFP, HIV-GFPNevR, and R7X4-GFP. The half-life of viral fusion for HIV-GFP and R7X4-GFP was calculated and averaged. SE is in parenthesis. Normalized half-life of uncoating was calculated by subtracting the half-life of fusion from uncoating for HIV-GFP and R7X4-GFP. ND, not determined.