Fig. 2.

Reduction in CF-mediated Ca²⁺ transients in PCs of PC-Ca_v2.1 KO mice. (A and B) Simultaneous whole-cell recordings and two-photon Ca²⁺ imaging from PCs of control (A) and PC-Ca_v2.1 KO (B) mice in vivo. (Left) Horizontal (Upper) and parasaggital (Lower) projection images of recorded PCs at P7. (Right) Somatic Ca²⁺ transients (Top) and membrane potential (Middle) were recorded simultaneously. Note that trains of burst firing were often observed (Bottom). (C) Average firing rate of burst firing. (D) Somatic and dendritic Ca²⁺ elevation evoked by each train of burst firing was quantified as (area of Ca²⁺ transients)/(number of burst firings in train). *P < 0.002; **P < 0.0001.