Table 1.
Summary of Gene Probes with Positive Hybridizations to the Control Strains and Test Isolates
| Aminoglycoside | Beta-lactam | Chloramphenicol | Efflux | Glycopeptide | Heavy metal | Macrolide | Metronidazole | Poly-ketide | Quaternary ammonium | Sulfonamides | Tetracycline | Transfer associated | Trimethoprim | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| LT2 | aadA1 | acrR, emrR, marA, marB | nitroimidazole resistance | |||||||||||
| CT18 | aac(3), aadA, strA,strB | bla, blaPER2, blaTEM-1, tem | cat, cat4, catIII | acrR, corA, emrR, marA, marB, msrA | ble | merACDRT | sulII | tet(A), tet(A)(B), tet(R) | htdAFT, InsA, IntI, parB, putative transposase, repC, repE, stbA, tnpA, tnpAIS26, tnpR, traI, trhBCFNUV | dfrA, dfrV, dfrV, dfrB | ||||
| SE Tc JF201 | aac(3), aadA, aadA, aadAb, aadA2*, aadA2, aadB, aadE, acc(6)-Ib*, aph(3)-I, aph2, aphA7, aphAI, ksgA, ntpII, strA*, strB, str3K | ampC, ampR, blaL2, bla, PER2, cmy-2*, sme-1*, tem | cat, cmlA5, flo* | acrR, corA, EmrA, emrR, marA, marB, msrA, ABC transporter, ybit | ble | merACFT | nonR | qac, qacEdelta | sul, sulII | tet(A), tet(A)(B), tet(C), tet(D), tet(R) | InsA, IntI, putative transposase, tnpA, tnpAIS26, tnpM, tnpR | dfr, dfr6, dfrA | ||
| SE Hd JF210 | aac(3), aadA, aadA, aadAb, aadA2*, aadA2, aadA7, aadB, acc(6)-Ib*, aph(3)-I, aphA7, aphAI, ksgA, ntpII, orfE, SAT-2, strA*, strB, str3K | ampC, bla, blaL2, blaTEM-1, cmy-2*, sme-1*, tem | cat, cmlA5, flo* | acrR, corA, emrR, marA, marB, msrA, ABC transporter, ybit | ble | merACDFPRRT, orf5 | nonR | qac, qacEdelta | sul, sulII | tet(A), tet(A)(B), tet(C), tet(D), tet(R) | InsA, IntI, mobA, putative transposase, tnpA, tnpAIS26, tnpM, tnpR,trhCF | dfr, dfr6, dfrA | ||
| EC JF220 | aac(3), aac(6), aadA, aadAb, aadA2*, aadE, aph(3)-I, aphA7, aphAI, ksgA, strA*, strB, str3K | ampC, ampR, bla, blaL2, blaTEM-1, cfxA, cmy-2*, penA, sme-1*, tem | flo* | acrR, corA, EmrA, emrR,marA, marB, msrA, potassium-transporting ATPase, ABC transporter, ybit | ble, vanH | merACDFPRRT, orf5 | nonR | qac, qacEdelta | sul, sulII | tet(A), tet(C), tet(D), tet(R) | InsA, IntI, putative transposase, tnpA, tnpAIS26, tnpM, tnpR | dfrA, dhf | ||
| EC JF227 | aacC3, aadA, aadAb, aadA2*, aadE, aph(3)-I, aphA7, aphAI, ksgA, strA*, strB, str3K | ampC, ampR, bla, blaL2, blaTEM-1, cfxA, cmy-2*, sme-1*, tem | cat, flo* | acrR, corA, EmrA, emrR, marA, marB, msrA, potassium-transporting ATPase, ABC transporter, ybit | ble, vanH | merADFRRT, orf5 | nonR | qac, qacEdelta | sul, sulII | tet(A), tet(A)(B), tet(C), tet(D), tet(R) | InsA, IntI, putative transposase, tnpA, tnpAIS26, tnpM | dfrA, dhf | ||
| CC 14-22 | aad9, aphA-3 | cam, cfiA | cmeBC, hsdR, marR | tet(O) | btgA, trfA2, trhU | |||||||||
| CC 141-27 | aphA-3 | blaL2, cam | cmeBC, hsdR, potassium-transporting ATPase | tet(O) | htdT | |||||||||
| ECF ATCC25788 | vanC* | merT | tnpA, trans | |||||||||||
| EGM ATCC49573 | blaL2, MecR | acrR, EmrA, potassium-transporting ATPase, ABC transporter, ybit | ble, vanC* | tet(M), tet(O), tet(S) | putative transposase, tnpA, tnpR, trans | |||||||||
| LI03 | msrC, potassium-transporting ATPase, ybit | ble | tet(M), tet(O), tet(S) | putative transposase, recP | ||||||||||
| LI04 | blaTEM-1 | msrC, potassium-transporting ATPase, ybit | ble | nitroimidazole resistance | tet(M), tet(O) | putative transposase | ||||||||
| MRSA G10-C87-2 | blaI, mecA, mecI, mecR, MecR, pre, penicillinase repressor, spc | marR, potassium-transporting ATPase | ble | ermA | putative transposase, tnpA, tnpAIS26, tnpB, tnpC, transposase B | |||||||||
| MRSA DN | aadE, aphA-3, sat4 | blaI, mecA, mecR, MecR, pre, penicillinase repressor, spc | cat | marR, potassium-transporting ATPase | ble | ermA | putative transposase, tnpA, tnpB, tnpC, transposase B | |||||||
| MRSA 36-1 | aadE, aphA-3, sat4 | blaI, mecA, MecR, pre | ermC | putative transposase, repC | ||||||||||
| MRSA 59 | aadE, aphA-3, sat4 | mecA, MecR | putative transposase, repC | |||||||||||
| CD 70 | ||||||||||||||
| CD 98 | orfE | |||||||||||||
| CD 112 | blaL2 | tet(C) | parB, tnpB, tnpR, trhC | |||||||||||
| CD 113 |
Complete data in Supplemental Table S1 (available online at www.liebertonline.com/mdr).
*
Indicates multiple identical probes for this gene. Genes with more than one different probe are listed for each positive hybridization detected.
Isolates: SE, Salmonella enterica; Hd, Heidelberg; Tc, serovar Typhimurium variant Copenhagen; EC, Escherichia coli; CC, Campylobacter coli; ECF, Enterococcus casseliflavus; EGM, Enterococcus gallinarum; LI, Listeria Innocua; MRSA, Methicillin Resistant Staphylococcus aureus; CD, Clostridium difficile.