Table 3.
LC-MS-MS analysis of sphingolipid composition in total serum fractions from CCHS serum samples.
| Sphingolipid1 | Concentration (nM) | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| High HDL-C, IHD2 | High HDL-C, No IHD2 | Low HDL-C, IHD2 | Low HDL-C, No IHD2 | |||||||
| Mean | Std | Mean | Std | P value3 | Mean | Std | Mean | Std | P value3 | |
| S1P | 1889.9 | 546.0 | 1849.8 | 407.6 | O-NS | 1879.2 | 549.0 | 2053.0 | 628.0 | O-NS |
| Sph | BDL | 22.3 | 11.0 | NA | 16.0 | 11.6 | 52.3 | 59.9 | <0.0001 | |
| DHSph | BDL | 14.8 | 9.2 | NA | 10.1 | 5.1 | 16.6 | 11.6 | <0.0001 | |
| DH-S1P | 213.0 | 76.1 | 276.8 | 96.5 | 0.0032 | 273.9 | 98.2 | 271.3 | 106.0 | 0.9992 |
| C16-Cer | 171.4 | 70.7 | 212.0 | 86.8 | 0.0276 | 360.1 | 163.9 | 222.5 | 78.7 | <0.0001 |
| DHC16-Cer | 139.1 | 74.9 | 60.1 | 38.5 | <0.0001 | 77.1 | 39.7 | 75.5 | 31.6 | 0.9958 |
| C14-Cer | 54.6 | 8.9 | 92.3 | 19.7 | <0.0001 | 117.0 | 34.4 | 103.0 | 35.1 | 0.0412 |
| C-18:1-Cer | 91.6 | 33.3 | 49.5 | 13.5 | <0.0001 | 63.7 | 25.6 | 65.2 | 13.6 | 0.5492 |
| C18-Cer | 163.8 | 51.0 | 123.9 | 42.3 | 0.0002 | 171.2 | 69.9 | 123.2 | 31.9 | <0.0001 |
| C20:1-Cer | 57.5 | 38.5 | 17.4 | 6.1 | <0.0001 | 28.4 | 15.4 | 25.0 | 9.7 | 0.9997 |
| C20-Cer | 481.1 | 205.9 | 249.9 | 94.2 | <0.0001 | 320.9 | 153.3 | 270.8 | 120.0 | 0.3547 |
| C22-Cer | 1045.2 | 347.4 | 758.2 | 194.0 | <0.0001 | 1116.0 | 420.3 | 915.4 | 317.9 | 0.0147 |
| C20:4-Cer | BDL | BDL | NA | BDL | 6.7 | 10.1 | NA | |||
| C22:1-Cer | 128.7 | 118.9 | 62.3 | 37.3 | 0.0061 | 206.5 | 160.1 | 61.4 | 47.9 | <0.0001 |
| C24-Cer | 6026.3 | 1816.2 | 5522.0 | 1381.9 | O-NS | 5577.5 | 2080.1 | 5724.1 | 1715.1 | O-NS |
| C24:1-Cer | 3433.4 | 847.9 | 2424.3 | 545.8 | <0.0001 | 2838.6 | 951.2 | 3223.2 | 688.7 | 0.0681 |
| C26-Cer | 40.7 | 32.8 | 33.8 | 15.7 | O-NS | 38.9 | 30.2 | 36.0 | 18.8 | O-NS |
| C26:1-Cer | 30.7 | 20.0 | 30.8 | 11.9 | 0.8093 | 40.3 | 21.2 | 29.8 | 15.9 | 0.0140 |
1Abbrevations used: Sph, sphingosine; DH, dihydro; Cer, ceramide; P, phosphate; BDL, below detection limit; O-NS, Overall not significant by ANOVA; NA, not applicable since at least one group in the pair was below detection limit.
2Sample sizes are High HDL-C, IHD, n = 53; High HDL-C, no IHD, n = 55; Low HDL-C, IHD, n = 42; Low HDL-C, no IHD, n = 54.
3p-values are derived from a Tukey's comparison following a one-way ANOVA test conducted at level of significance 0.05.