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Primaquine inhibits the rapid LPS-induced increase in cell-surface MHC class I molecules. Day 7 cluster purified immature DC were incubated with LPS alone (I), LPS and brefeldin A (II), and LPS and primaquine (III). Cells were harvested at 0, 5, and 24 h after addition of the LPS and fixed; expression of MHC class I molecules was examined by flow cytometry.
