Table 2.
Primers, PCR reaction conditions, and characteristics of microsatellite loci tested for amplification success in Asclepias syriaca
| Primer Set Name | Orientation | Primer Sequence (5'-3') | Annealing Temp. (°C) | Repeat Motif | Product Size (bp) |
|---|---|---|---|---|---|
| As104467 | FORWARD | ACTTTTTGTTCTGTTCCGAGT | 52.9 | TA6 | 100 |
| REVERSE | CGGGGCTAGTATAGAAGAAGA | ||||
| As106994 | FORWARD | AGGAAGCCAATTCAAGTAAAG | 48.9 | GA5 | 129 |
| REVERSE | GAAAATCCTTGCATGAAACT | ||||
| As12122 | FORWARD | TTGGTTGTTGGAACTTACACT | 50.4 | ATTC4 | 115 |
| REVERSE | AAGCACAAGAATCAGAACAAT | ||||
| As125636 | FORWARD | GAACTCCTATGATCTTTCATTT | 50.8 | TC4...TA4* | 136 |
| REVERSE | AGACATCATGAACCCTCTTTA | ||||
| As12641 | FORWARD | CTCTTCATCTGGTTTCTCTTG | 49.0 | TA4 | 130 |
| REVERSE | TGCATACAAATTTAGGTGATTC | ||||
| As13110 | FORWARD | AACCATAAATGCAGGTTCTTT | 50.9 | TA4 | 101 |
| REVERSE | GCATATCTAGCTCGCATTTAG | ||||
| As16135 | FORWARD | TTAGGTTGTGAGAAGGGATTT | 50.3 | CCT4 | 100 |
| REVERSE | GCTTTTATTAATGTGGGAGGA | ||||
| As16369 | FORWARD | AACGCTTATCCCTCACTTTAC | 51.5 | TA5 | 100 |
| REVERSE | CCGAACTCTATATGAGTCCAAT | ||||
| As196582 | FORWARD | GCATTTATTGCATTGTCAAA | 50.5 | TGA5 | 113 |
| REVERSE | GCCGTTGTTCCTCTATAATTT | ||||
| As2003a | FORWARD | CCCTCGTTATTGAGAAAATAGA | 50.5 | ATA5 | 105 |
| REVERSE | GGCTCTTACCAAAGAAAAGAT | ||||
| As20679a | FORWARD | GTCATCGGAAGCTCTTTTC | 52.7 | CTT4 | 104 |
| REVERSE | AGTCAAAAGTCACACTCATCTG | ||||
| As2338 | FORWARD | CATCTTGGTTAGCTCGTAAAC | 51.7 | TC5 | 104 |
| REVERSE | CCTTACCTAGGATGAAAGGAG | ||||
| As23491a | FORWARD | ATTTCCTCCATTTGTTCTTTC | 50.0 | TC5 | 101 |
| REVERSE | GAAGTTTGTCTGAAATGGTTG | ||||
| As2366 | FORWARD | TTGGTGTTGTAAAGTGTTCCT | 54.5 | TA5 | 101 |
| REVERSE | ACCAGCTCGTCAGTCAAG | ||||
| As23942 | FORWARD | GTGATTTCCATTTTACCATACC | 51.7 | AG5 | 100 |
| REVERSE | ATCCTTAGCAAATAGGAGTCG | ||||
| As23961 | FORWARD | ACTCTTGTTATTTTGGGGAAT | 49.3 | TG5 | 108 |
| REVERSE | CAAAAGCTTTACACGATCAAT | ||||
| As282930 | FORWARD | AACTTAGCTTTGGATGATGTTT | 50.1 | TAA4 | 102 |
| REVERSE | CAATTGGGCTTGTATATCAGA | ||||
| As28460 | FORWARD | CACAAGATGAACATCAAAACC | 49.6 | AT5 | 100 |
| REVERSE | TTGAGTATATTTGTTCCCCAAT | ||||
| As292191 | FORWARD | GGAGGGTCTAATGTCTGATGT | 51.3 | TG5 | 125 |
| REVERSE | ATTCACACATCCCATACCAT | ||||
| As43163 | FORWARD | TATTCCAAGAAATTGGTCAAC | 49.7 | TTA4 | 110 |
| REVERSE | CATGGAAGAAGAACAAAACAG | ||||
| As5768 | FORWARD | AAGGGAAATGATCCTTAGGTA | 51.0 | AG6 | 108 |
| REVERSE | TTCGAAAGAGTTCCACATCTA | ||||
| As71725 | FORWARD | TTTACGACTCATGTACTCTTCC | 50.8 | AG4...TG5* | 133 |
| REVERSE | GTAATACCGGAAAATGACCTC | ||||
| As77693 | FORWARD | GTCAGTCAACTCAACAATGCT | 51.0 | TC6 | 102 |
| REVERSE | TCCCAAAACTCAGATCTAACA | ||||
| As81784 | FORWARD | AATGTAAAGGGAGATGTTTATCA | 52.0 | TAT4 | 129 |
| REVERSE | AGCATCAAGAACTTGAACAGA | ||||
| As99212 | FORWARD | TCTATGGTCAACAATTTCTGC | 53.4 | AT5 | 153 |
| REVERSE | TTTATAGGGGTAGCGTAGGTG | ||||
*Repeat motifs are separated by 27 bp for primer set As71725 and 46 bp for primer set As125636.