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. Author manuscript; available in PMC: 2011 Dec 1.
Published in final edited form as: Clin Proteomics. 2010 Dec;6(4):129–151. doi: 10.1007/s12014-010-9055-y

Fig. 3.

Fig. 3

Changes in total and phosphoproteins with increasing time to breast tumor freezing. Six western blots demonstrate stability of mitogen-activated protein kinase (ERK2), Akt, and AMP-activated protein kinase (AMPK) expression and of Akt phosphorylation (Aktp473) with increasing time to tumor freezing. In contrast, consistent with RPPA data, a progressive deterioration was seen with increasing time to breast tumor freezing in the phosphorylation of mitogen-activated protein kinase (MAPKp) and in the phosphorylation of AMPK (AMPKp). The time before tumor freezing is shown along the top of the figure