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. 2011 Mar-Apr;1(2):57–68. doi: 10.4161/cl.1.2.15289

Figure 2.

Figure 2

Rab11-FIP2 mutants alter the distribution of clathrin heavy chain. Confocal fluorescence microscopic images of polarized MDCK cells. X-Y plane images are shown flanked by X-Z projections (horizontal) and Y-Z projections (vertical). (A) Parental T23 MDCK cells were stained with Alexa-488-phalloidin (pseudo-colored green) to show the cell boundaries and clathrin heavy chain (pseudo-colored red) and imaged by confocal microscopy. Punctate clathrin staining was observed on vesicles throughout the cytoplasm. (B) T23 MDCK cells stably expressing EGFP-Rab11-FIP2(SARG) and EGFP-Rab11-FIP2(ΔC2) were stained for endogenous clathrin heavy chain (pseudo-colored red) and imaged by confocal microscopy. Clathrin heavy chain staining was accumulated with the EGFP-Rab11-FIP2(SARG) mutant in its collapsed cisternum. In EGFP-FIP2(ΔC2)-expressing cells, clathrin staining was observed predominantly as vesicles surrounding the Rab11-FIP2 containing membranes. Images were taken with a 100x lens with a 3x zoom. Scale bars represent 5 µm.