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. 2011 Mar-Apr;1(2):57–68. doi: 10.4161/cl.1.2.15289

Figure 3.

Figure 3

Rab11-FIP2 mutants alter AP-1 distribution. Confocal fluorescence microscopic images of polarized MDCK cells. X-Y plane images are shown flanked by X-Z projections (horizontal) and Y-Z projections (vertical). (A) Parental T23 MDCK cells were stained with Alexa-488-phalloidin (pseudo-colored green) to show the cell boundaries and antibodies against AP-1 (pseudo-colored red) and imaged by confocal microscopy. AP-1 staining vesicles were observed diffusely within the subapical cytoplasm. (B) T23 MDCK cells stably expressing EGFP-Rab11-FIP2(SARG) or EGFP-Rab11-FIP2(ΔC2) were stained for endogenous AP-1 (pseudo-colored red) and imaged by confocal microscopy. AP-1 showed a prominent accumulation with EGFP-Rab11-FIP2(SARG) containing cisternae. In Rab11-FIP2(ΔC2)-expressing cells, AP-1-containing vesicles were clustered around the Rab11-FIP2-containing cisternum. Images were taken with a 100x lens with a 3x zoom. Scale bars represent 5 µm.