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. 2011 Mar-Apr;1(2):57–68. doi: 10.4161/cl.1.2.15289

Figure 5.

Figure 5

Differentiable influences Rab11-FIP2 mutants on mRFP-Rab5b. Confocal fluorescence microscopic images of polarized MDCK cells. X-Y plane images are shown flanked by X-Z projections (horizontal) and Y-Z projections (vertical). Parental MDCK-T23 cells and cells overexpressing EGFP-Rab11-FIP2 constructs were transiently transfected with mRFP-Rab5b. Top part: In parental T23 cells, mRFP-Rab5b was distributed in fine punctate structures throughout the cells. Lower parts: T23 MDCK cells stably expressing wild-type EGFP-FIP2 and each of the mutants (EGFP-FIP2(SARG) and EGFP-FIP2(ΔC2)) were transiently transfected with mRFP-Rab5b and imaged by confocal microscopy. Overexpression of either wild-type Rab11-FIP2 or Rab11-FIP2(SARG) had no effect on the distribution of Rab5b. However, overexpression of EGFP-FIP2(ΔC2) caused a redistribution of mRFP-Rab5b-containing vesicles towards the Rab11-FIP2 containing membrane cisternae. Nevertheless, little direct co-localization was observed. Images were taken with a 100x lens with a 3x zoom. Scale bars represent 5 µm.