Figure 3.

Garz depletion inhibits surface delivery of adhesion proteins and disrupts development of salivary glands in Drosophila. Salivary glands were dissected from control or Garz-depleted third instar (late L-3) larva, processed for IF with the indicated antibodies and the nuclei were stained with Hoechst (A and B). Salivary glands from control or Garz-depleted third instar larva expressing actin-GFP transgene were stained with Hoechst and imaged (C). Bars, 100 µm. Insets in (A) show enlarged areas. Garz depletion inhibits trafficking of DE-cad and Flamingo adhesion proteins to cell surface (A), disrupts localization of Dlg (B) and causes disruption in salivary gland development (C).