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. 2001 Mar 27;98(7):3988–3991. doi: 10.1073/pnas.071050898

Figure 2.

Figure 2

Clonality of PM1#11 crossreacting response to GAD65 and hCMV. T cells were prepulsed overnight with either GAD65 339–352 or hCMV major DNA-binding protein 674–687 (10 μM). After washing the T cells, cells were stimulated with DR3-matched irradiated peripheral blood mononuclear cells and either GAD65 339–352 or hCMV major DNA-binding protein 675–687 (1 μM) and cultured as described. As a control for the ability to proliferate, prepulsed cells were stimulated with IL-2. In a negative control experiment (data not shown), PM1#11 was mixed with a coxsackie B4 virus-specific T cell clone and prepulsed with either the coxsackie B4 virus-derived epitope or GAD65 339–352. The response of the T cell mixture, after washing, to GAD65 339–352 was completely abrogated after prepulsing with GAD65 339–352, but not after prepulsing with coxsackie B4A virus-derived epitope. In all experiments, responses are the means ± SD of triplicate tests. Mean background of triplicates after prepulsing never exceeded 500 cpm.