Figure 1.

Localization of active RhoA in crawling and transmigrating T cells. Schematics show a T cell crawling on the apical surface of EC (viewed from above) (A) or transmigrating between adjacent EC (viewed from the side) (B). RhoA is active in the uropod (red) in both the crawling (A) and transmigrating T cell (B). In the crawling T cell (A), RhoA is also active in lamellipodia (green) where it is associated with both extension and retraction events (dotted arrows), and in dynamic puncta on the basal membrane (dark blue dots). During transmigration (B), RhoA is active at the leading edge of the T cell in filopodia (pale blue regions), lamellipodia (green) and in dynamic puncta (dark blue dots).