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. Author manuscript; available in PMC: 2012 Jun 1.
Published in final edited form as: Anal Chem. 2011 May 5;83(11):4110–4117. doi: 10.1021/ac200253x

Figure 4.

Figure 4

Sample preparation schematic and assay enhancement ratio as a function of initial off-chip incubation time, tH. (a) Schematic of sample lysis and dilution. A pellet (from either bacterial culture or infected patient urine) is lysed at room temperature for 5 min. The lysate is diluted 10× with buffer containing molecular beacons and MgCl2. The mixture of sample and beacons is then incubated at either room temperature or 60°C. 2 μL are introduced into the microfluidic chip reservoir. (b) Enhancement ratio as a function of off-chip hybridization time, using a bacterial culture sample. Shown are pre-ITP incubations at either room temperature or for 5 min at 60°C followed by incubation at room temperature. There is a tradeoff between total assay time and sensitivity. Based on these data, we chose off-chip, pre-ITP incubation at 60°C for 5 min. The assay can be completed in under 15 min from beginning of lysing to signal detection.