Skip to main content
. 2001 Mar 27;98(7):3992–3997. doi: 10.1073/pnas.071523398

Figure 1.

Figure 1

Characterization of the Meth A-specific CD4+ line and a representative clone 24D3 derived from it. (A) TML-01 proliferates in response to lysates of Meth A but not other tumors and normal tissues indicated. TML-01 cells (2 × 104 cells) were cultured in the presence of 5 × 105 irradiated BALB/c splenic APCs with or without lysate of tumors or normal fetal fibroblast (ff) (5 × 103 cell equivalents per milliliter) for 4 days. [3H]Thymidine (0.5 μCi per well) was added 16–18 h before harvesting and counting. (B) Antigen-specific proliferation of TML-01 is blocked by anti-CD4 (PharMingen) but not anti-CD8 monoclonal antibody (PharMingen) and by anti-I-Ed (Accurate Chemicals) but not anti-I-Ad monoclonal antibody (PharMingen). (C) CD4+ T cell clone 24D3, derived from TML-01, is stimulated in response to lysates of Meth A but not other tumors indicated. IL-5 secretion was measured in the supernatant of day 2 culture by ELISA (Endogen). (D) Stimulation of clone 24D3 is blocked by anti-CD4 but not anti-CD8 monoclonal antibody and by anti-I-Ed but not anti-I-Ad monoclonal antibody.