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. 2001 Mar 27;98(7):3992–3997. doi: 10.1073/pnas.071523398

Figure 3.

Figure 3

Molecular characterization of mouse L11 and of a point mutation in L11 in Meth A. (A) Sequence of cDNA encoding L11 from a CD4+ T cell clone (as normal control) and Meth A compared with human (29) and rat (30) L11 cDNA. The sequences in the normal control and Meth A were identical except for an A-to-C substitution at base pair 289 and a corresponding amino acid substitution of N by H change at amino acid 97. The Meth A contained both the normal and mutated sequences. (B) Base pairs 271–300 of L11 cDNA from Meth A, CMS4, and CMS5 were amplified by PCR. Cloned normal or mutated PCR products derived from the Meth A L11 sequence were also sequenced. Direct sequencing of amplified product derived from Meth A shows double sequences (A and C) at position 289. Direct sequencing of amplified products of CMS4 and CMS5 tumors shows only a single sequence A at that position. Sequences of cloned L11 PCR products derived from Meth A show normal or mutated sequence A or C at position 289.