Figure 5.
Immunogenicity of the mutated L11 peptide in vivo. (A) BALB/cJ mice were immunized s.c. twice one week apart with PBS or 10 μg of polysome (prepared as described in ref. 31) derived from normal liver or from Meth A. Mice were challenged intradermally with 1 × 105 Meth A cells 1 week after the last immunization. The kinetics of tumor growth in individual mice is shown. (B) Mice were immunized with PBS, or the wild type or mutant 19-mer peptide (100 μg peptide/PBS emulsified with an equal volume of incomplete Freund's adjuvant per mouse) and were challenged as in A. The kinetics of tumor growth in individual mice is shown. (C) Adoptive transfer of clone 24D3 into BALB/cJ mice protects them against Meth A but not antigenically distinct CMS5 sarcoma. Mice received medium alone or 1 × 107 cells of CD4+ T cell clone 24D3 by adoptive transfer (32). Mice were challenged with 1 × 105 Meth A or 2 × 105 CMS5 cells intradermally, 3 h after adoptive transfer. The kinetics of tumor growth in individual mice is shown. Tumor volume (mm3) = 0.4 × (longest diameter) × (shortest diameter)2 (33). P values were calculated by Student's t test.