Table 3.

Schematic summary of the histological results for all the groups and for each skin layer.

Time of exposure and skin layers		T (Control untreated group) 20 explants	F (HF without washing) 18 explants	FWCaG (HF + water washing + calcium gluconate) 16 explants	FHexa (HF + Hexafluorine® 400 mL) 16 explants
T0	Epidermis	GM = good morphology	GM	GM	GM
	Papillary dermis
	Reticular dermis
20 s	Epidermis
	Papillary dermis
	Reticular dermis
5 min	Epidermis		PN + AC
	Papillary dermis
	Reticular dermis		PN + CA moderately
10 min	Epidermis		PN= pyknotic nucleiAC = acidophilic cytoplasm
	Papillary dermis
	Reticular dermis
15 min	Epidermis			PN + AC moderately
	Papillary dermis			PN + AC
	Reticular dermis
30 min	Epidermis			Some necrotic cells
	Papillary dermis			GM
	Reticular dermis
1 h	Epidermis
	Papillary dermis
	Reticular dermis
2 h	Epidermis
	Papillary dermis
	Reticular dermis
4 h	Epidermis			Slightly edematous cells with mild acantholysis
	Papillary dermis			GM
	Reticular dermis
24 h	Epidermis		Totally necrotic	Very edematous cells with a very clear cytoplasm
	Papillary dermis		PN + AC	PN + AC
	Reticular dermis			Lesser alterations

GM, good morphology; PN, pyknotic nucleus; AC, acidophilic cytoplasm.

PN and AC are described as part of the appearance of coagulation necrosis.

In all treatment groups, 70% HF saturated filter paper disks were left in contact for 20 s and then removed.