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. Author manuscript; available in PMC: 2012 May 1.
Published in final edited form as: Int J Parasitol. 2011 Feb 15;41(6):595–607. doi: 10.1016/j.ijpara.2010.11.012

Fig. 8.

Fig. 8

Toxoplasma gondii inhibits apoptosis and granzyme B (GrB) activity after delivery by pinocytic lysis. Jurkat cells were infected with T. gondii expressing yellow fluorescent protein (A) or expressing mCherry (B) at a multiplicity of infection of 1 for 16 h. The frequencies of infection were 26% and 45%, respectively. GrB (60 nM), in the absence of endosomolytic agents, was loaded into cell cytosol by osmotic lysis of pinosomes. (A) At 2.5 h after GrB loading, the cells were analyzed by flow cytometry for apoptosis by annexin staining. Uninfected and infected fractions of the same culture were compared. * P < 0.001 compared with uninfected. (B) After pinocytic loading, cells were washed, suspended in medium and allowed to recover for 30 min. GranToxiLux (1/3 volume) was added and after 2 h the cleavage of GranToxiLux was analyzed by flow cytometry. Uninfected (Uninf.) and infected (Inf.) fractions of the same culture were compared. * P < 0.05 compared with uninfected. The data are representative of two experiments.