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. 2011 Jun 16;7(6):e1002072. doi: 10.1371/journal.ppat.1002072

Figure 10. Alignment and functional characterization of Nb_An05 mutants.

Figure 10

(A) Alignment of the Nb_An05 mutant sequences with the wild type sequence; only the mutations in the complementarity determining regions are shown. (B) Flow cytometry analysis using wild type Nb_An05 and derived variants (Nb_An05-2,4,5,6,12,17,19), detected with mouse anti-6⋅His IgG and a phycoerythrin-labeled rat anti-mouse IgG (blue line). The black line represents controls with no Nb. For each experiment 2×105 parasites were incubated with 1 µg Nb (VSG∶Nb molar ratio of 1∶20).