Skip to main content
. 2011 Jun 16;7(6):e1002084. doi: 10.1371/journal.ppat.1002084

Figure 6. SUMO accumulation at sites associated with incoming viral genomes is not dependent on RNF8 or RNF168.

Figure 6

(A) Control cells or cells in which RNF8 had been depleted using shRNA were infected with ICP0-null HSV-1 at an MOI of 0.1 for 1 hr. Cells were fixed at 24 hpi, stained for ICP4, and localization of SUMO1 or SUMO2/3 was assessed in asymmetrically infected cells. (B) RIDDLE cells or RIDDLE cells with HA-tagged RNF168 reconstituted were infected and analyzed as in A. (C) Control HFF cells or cells in which RNF8 had been depleted using shRNA were infected as in A and assessed for localization of PML. (D) RIDDLE cells or RIDDLE cells with HA-tagged RNF168 reconstituted were infected and analyzed as in C.