Figure 2. Production of progeny virus in CD36⁺ EPCs under hypoxia vs. normoxia.

Day 8 CD36⁺ EPCs were infected with B19V at a low MOI (1,000 gc/cell) under either hypoxia or normoxia. At 72 hrs p.i., infected cells were lysed by repeated freeze-thaw cycles, to release progeny virus. After a brief spin, one third of the lysate from each culture was used to infect fresh day 8 CD36⁺ EPCs cultured under the same conditions. (A&B) At each passage, infected cells were examined by immunofluorescence to detect staining with an anti-B19V capsid antibody (detection for assembled capsids). (C&D)The production of progeny virus was quantified by qPCR-based assessment of the number of copies of the viral genome (C) and assessed for infectious units by titration of ffu (D).