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. 2011 Jun 16;6(6):e20740. doi: 10.1371/journal.pone.0020740

Table 1. Characteristics of the UCB units after EloHAES separation and cryopreservation.

Collected UCB Volume reduced UCB Thawed UCB
Volume NCs NCs CD34+ cells NCs CD34+ cells Recovery CD34+ cells
ml x106 x106 x106 x106 x106 %
Donor 1 88 1294 790 3.90 368 2.96 76
Donor 2 151 1857 1312 5.88 469 3.73 63
Donor 3 141 1734 1378 4.96 653 3.23 65
Donor 4 87 1992 1588 8.72 819 8.12 93
Donor 5 119 1821 1106 3.68 583 2.28 62
Donor 6 153 1775 1519 3.17 829 2.15 68
Donor 7 152 1733 978 2.08 440 2.06 99
Donor 8 72 1210 760 2.70 403 2.07 77
Donor 9 78 772 600 3.96 248 1.84 46
Donor 10 97 927 616 1.73 386 1.69 98
Donor 11 81 1207 974 2.82 479 2.52 89
Donor 12 175 2380 1721 6.90 943 3.96 57
Donor 13 95 1430 1008 3.04 558 2.66 87
Donor 14 77 857 680 1.75 273 1.43 82
Donor 15 88 1223 969 2.40 563 2.14 89
Donor 16 130 1829 1364 2.78 821 1.82 66
mean 111 1503 1085 3.78 552 2.79 76
SD 34 455 357 1.95 210 1.59 16
median 96 1581 993 3.11 518 2.21 76
min 72 772 600 1.73 248 1.43 46
max 175 2380 1721 8.72 943 8.12 99

The table summarizes the processing of 16 UCB units used for CD34+ enrichment after collection, volume reduction and thawing process. Nucleated cells (NCs) were counted with the AcT10 counter (Beckman coulter). CD34+ cells were enumerated by single platform flow cytometry analysis. Results are depicted as mean, standard deviation, median, minimum (min) and maximum (max) volume, number of cells or percentages, respectively.