Table 1. Characteristics of the UCB units after EloHAES separation and cryopreservation.
Collected UCB | Volume reduced UCB | Thawed UCB | |||||
Volume | NCs | NCs | CD34+ cells | NCs | CD34+ cells | Recovery CD34+ cells | |
ml | x106 | x106 | x106 | x106 | x106 | % | |
Donor 1 | 88 | 1294 | 790 | 3.90 | 368 | 2.96 | 76 |
Donor 2 | 151 | 1857 | 1312 | 5.88 | 469 | 3.73 | 63 |
Donor 3 | 141 | 1734 | 1378 | 4.96 | 653 | 3.23 | 65 |
Donor 4 | 87 | 1992 | 1588 | 8.72 | 819 | 8.12 | 93 |
Donor 5 | 119 | 1821 | 1106 | 3.68 | 583 | 2.28 | 62 |
Donor 6 | 153 | 1775 | 1519 | 3.17 | 829 | 2.15 | 68 |
Donor 7 | 152 | 1733 | 978 | 2.08 | 440 | 2.06 | 99 |
Donor 8 | 72 | 1210 | 760 | 2.70 | 403 | 2.07 | 77 |
Donor 9 | 78 | 772 | 600 | 3.96 | 248 | 1.84 | 46 |
Donor 10 | 97 | 927 | 616 | 1.73 | 386 | 1.69 | 98 |
Donor 11 | 81 | 1207 | 974 | 2.82 | 479 | 2.52 | 89 |
Donor 12 | 175 | 2380 | 1721 | 6.90 | 943 | 3.96 | 57 |
Donor 13 | 95 | 1430 | 1008 | 3.04 | 558 | 2.66 | 87 |
Donor 14 | 77 | 857 | 680 | 1.75 | 273 | 1.43 | 82 |
Donor 15 | 88 | 1223 | 969 | 2.40 | 563 | 2.14 | 89 |
Donor 16 | 130 | 1829 | 1364 | 2.78 | 821 | 1.82 | 66 |
mean | 111 | 1503 | 1085 | 3.78 | 552 | 2.79 | 76 |
SD | 34 | 455 | 357 | 1.95 | 210 | 1.59 | 16 |
median | 96 | 1581 | 993 | 3.11 | 518 | 2.21 | 76 |
min | 72 | 772 | 600 | 1.73 | 248 | 1.43 | 46 |
max | 175 | 2380 | 1721 | 8.72 | 943 | 8.12 | 99 |
The table summarizes the processing of 16 UCB units used for CD34+ enrichment after collection, volume reduction and thawing process. Nucleated cells (NCs) were counted with the AcT10 counter (Beckman coulter). CD34+ cells were enumerated by single platform flow cytometry analysis. Results are depicted as mean, standard deviation, median, minimum (min) and maximum (max) volume, number of cells or percentages, respectively.