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Gal-3 mRNA levels were determined by quantitative real-time PCR (qRT-PCR) (A, B). Ribosomal protein, large, P0 (RPLP0) was used as the reference gene. PC: pancreatic cancer ; CP: chronic pancreatitis ; NP: normal pancreas. Protein expression in different pancreatic cancer cell lines was determined by Western blot (C) analyses. β-actin was used as loading control.
